99. Midterm Review
PCR Thermal Cycles
Description
Promoters
sites that signify the START of a gene RNA binds to this
What are Primers
Used to locate the gene of interest and isolate it from the rest of the DNA. Used in PCR
DNA Polymerase
Works from '3 to '5
Creates a strand 5' to 3'
-In Replication
Creates a strand 5' to 3'
-In Replication
DNA
DNA POLYMERASE
Description
Translation
End product: Protein
Translation
Decoded from 5' to 3'
Transcription
'5
Description
'3
Description
PCR equation
Amplification Errors:
Final DNA Concentration = Amplifier Gain x starting Concentration
Final number of DNA fragments after thermal cycles:
2^n-2*n
Final DNA Concentration = Amplifier Gain x starting Concentration
Final number of DNA fragments after thermal cycles:
2^n-2*n
Electrophoresis Equation
Force = qE = qV/d
E= Electrical Charge
V= Voltage
d= distance between electrodes
q= charge
E= Electrical Charge
V= Voltage
d= distance between electrodes
q= charge
Electrophoresis Equation 2`
Relative Distance along Gel = (1/2)(electrical force/fragment mass)(Gel resistance factor)(time^2)
OR
= (C/mass)
(the distance is inversely proportional to the mass)
OR
= (C/mass)
(the distance is inversely proportional to the mass)